Investigation of Oxidative Stress, Lipid Peroxidation and DNA Damage in Muscle Tissue of Young Trout Treated with Arsenic


Özet Görüntüleme: 16 / PDF İndirme: 9

Yazarlar

  • Aslı Yeltekin Van Yüzüncü Yıl Üniversitesi

DOI:

https://doi.org/10.5281/zenodo.12604250%20%20%20%20%20%20%20%20

Anahtar Kelimeler:

Arsenic, MDA, 8-OHdG, Antioxidants, Oxidative stress, Juvenile Rainbow Trout

Özet

Arsenic, metalloid and metal-based compounds commonly found in the earth's crust, dissolves through underground waters and causes toxic effects by spreading into industrial processes or the environment. In this study, modifies in the levels of antioxidant enzymes and some parameters (GSH-Px, CAT, MDA, SOD, and 8-OHdG) in the muscle tissues of juvenile trout (Oncorhynchus mykiss) after arsenic exposure were examined and the data obtained after the study were evaluated.

In our study, juvenile trout were divided into three groups and arsenic was applied to these three groups at saturation of 25, 50 and 75 mg/L. 96 hours after the application, the muscle tissues of the young trout were taken and homogenized. 8-OHdG levels in muscle tissue were determined using an ELISA kit. SOD, CAT, GSH-Px, and MDA levels were determined by spectrophotometric methods.

DNA damage was observed in juvenile trout with arsenic accumulation in muscle tissue, and accordingly, the 8-OHdG concentration level increased. Deterioration occurred in the membrane structure of the cell and lipid peroxidation occurred. When the muscle tissue of young trout treated with arsenic was examined, a significant decrease was observed in the concentrations of CAT, GSH-Px and SOD. It is thought that this situation is caused by oxidative stress caused by free radicals accumulating in the muscle tissue of fish. These changes were caused after the application of arsenic. The presence of heavy metals in large amounts in the living spaces of aquatic creatures negatively affects the life stage and developmental stages of the living creatures, and the presence of these creatures in the food chain and the use of these creatures as food by other species can cause significant toxicity in living creatures.

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Yayınlanmış

2024-07-01

Nasıl Atıf Yapılır

Yeltekin, A. (2024). Investigation of Oxidative Stress, Lipid Peroxidation and DNA Damage in Muscle Tissue of Young Trout Treated with Arsenic. International Journal of Medicine and Occupational Health and Safety Sciences, 2(1). https://doi.org/10.5281/zenodo.12604250

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